Assembly of dsDNA nanocircles into dimeric and oligomeric aggregates.
نویسندگان
چکیده
The assembly of double-stranded (ds) DNA nanocircles both by hybridization with branched oligodeoxynucleotides (ODNs) and by intercalation was analyzed by atomic force microscopy (AFM). Branched ODNs ligated to single-stranded (ss) gap regions of dsDNA nanocircles led to defined, dumbbell-shaped architectures. ODNs containing an aromatic intercalator yielded oligomeric aggregates.
منابع مشابه
Assembly of dsDNA nanocircles into dimeric and oligomeric aggregatesw
Nucleic acids are an ideal material for the construction of nanometre-scaled objects. DNA-based scaffolds of different topological and mechanical properties have been generated and applications for ordered nucleic acid nanoarchitectures have been implemented. Nucleic acids have also been used for building devices like walkers, tweezers, thermometers, or self-replicating systems, and for the dir...
متن کاملI-motif-programmed functionalization of DNA nanocircles.
The folding of various intra- and intermolecular i-motif DNAs is systematically studied to expand the toolbox for the control of mechanical operations in DNA nanoarchitectures. We analyzed i-motif DNAs with two C-tracts under acidic conditions by gel electrophoresis, circular dichroism, and thermal denaturation and show that their intra- versus intermolecular folding primarily depends on the le...
متن کاملSonication-induced self-assembly of flexible tris(ureidobenzyl)amine: from dimeric aggregates to supramolecular gels.
Tris(ureidobenzyl)amine derivatives 1a,b form dimeric aggregates in apolar solution and in the solid state. Specifically, the meta-substituted tris(urea) 1a is able to transform into supramolecular gels in certain solvents via sonication.
متن کاملSmall-angle neutron scattering reveals the assembly mode and oligomeric architecture of TET, a large, dodecameric aminopeptidase
The specific self-association of proteins into oligomeric complexes is a common phenomenon in biological systems to optimize and regulate their function. However, de novo structure determination of these important complexes is often very challenging for atomic-resolution techniques. Furthermore, in the case of homo-oligomeric complexes, or complexes with very similar building blocks, the respec...
متن کاملProbing of Amyloid Aβ (14-23) Trimers by Single-Molecule Force Spectroscopy.
Self-assembly and aggregation of amyloid peptides, such as Aβ(1-40) and Aβ(1-42), lead to the development of Alzheimer disease and similar neurodegenerative disorders associated with protein aggregation. The structures of large aggregates, specifically fibrils, are well characterized. However, our understanding about the structure of oligomeric forms of amyloids is incomplete and needs to be ex...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 46 23 شماره
صفحات -
تاریخ انتشار 2010